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The proteins were extracted with 1.2 ml NETT solution (1% Triton X-100, 2.5 M NaCl, 0.9 M TrisHCl [pH 7.5], 0.25 M EDTA, 100 mM PMSF), 200 L lysate was used as input whilst the remaining 1000 L samples were incubated with 30 L HA-tagged or Flag-tagged magnetic beads at 4 C overnight

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Intracellular ROS levels were detected by incubating cells with 10 M CM-DCFDA for 30 minutes, then analyzed by Accuri-C6 flow cytometry

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