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Enhanced expression of miR-21 resulted in a significant decrease in HMGCR mRNA and protein expression in HepG2 cells, leading to a reduction in TG and cholesterol levels [309]

Briefly, cells were lysed in PBS containing 1% Triton and 1 mM phenylmethylsulfonyl fluoride (PMSF), along with a complete set of EDTA-free protease inhibitors (Thermo Scientific, Rockford, IL, USA)

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The downstream normalization of nitric oxide and cytokine output may then secondarily interact with neighboring hepatocytes in cell cultures, including their IGF-I expression