Briefly, equal amount of samples were loaded onto 12% SDSPAGE gels, transferred to polyvinylidene difluoride (PVDF) membranes and analyzed using antibody against GPX4 at a concentration of 1:2,000
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This VDF cocktail induced high expression of key hepatic markers such as AFP, HNF4, and Albumin (ALB), with an efficiency of 95.5% (HNF4a+/AFP+ positive cells), closely matching that of the growth factor-based control
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TRPC6 contributes to the Ca(2+) leak of human erythrocytes