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Blots were incubated with HRP-conjugated secondary antibodies (BS13278, Bioworld Technology Inc., MN, USA) for 1 h, and protein expression was detected with ECL (WBULS0500, Millipore, MA, USA) by digital imaging systems (SYNGENE, Cambridge, UK)

Author Contributions Concept and design: Katie Hughes, Yovan Ram Kurrun Sumaruth, Elmahi Mohammed, Vibhootee Sant Bakshsingh Acquisition, analysis, or interpretation of data: Katie Hughes, Yovan Ram Kurrun Sumaruth, Elmahi Mohammed, Vibhootee Sant Bakshsingh Drafting of the manuscript: Katie Hughes, Yovan Ram Kurrun Sumaruth, Elmahi Mohammed, Vibhootee Sant Bakshsingh Critical review of the manuscript for important intellectual content: Katie Hughes, Yovan Ram Kurrun Sumaruth, Elmahi Mohammed, Vibhootee Sant Bakshsingh References 1.Clinical potential of treatment with semaglutide in type 2 diabetes patients