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HEK293T cells at roughly 60% confluency were infected with crude lentivirus followed by selection with 10 g ml 1 puromycin for 1 week

TA-OH exhibited blue fluorescence upon excitation at a wavelength of 315 nm

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Studies by our group have shown that both the ENTH domains of Ent1 (ENTH1) and Ent2 (ENTH2) can form the AENTH complex with yeast Hip1R homologue Sla2 28 coordinating Ent1 and Ent2 at the endocytic site 28