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After adding EnVision Flex 3,3-diaminobenzidine tetrahydrochloride (DAB, K346811-2, Agilent, Santa Clara, CA, USA) for 5 min at room temperature to visualize GPx3, the slides were washed with tap water, counterstained with hematoxylin and mounted in Pertex

Bipolar disorder: A landmark RCT (n=75) found that NAC (1,000 mg twice daily for 24 weeks) produced a significant improvement in depression scores (Montgomery-Asberg Depression Rating Scale) in bipolar disorder as an adjunct to usual treatment (Berk et al., Biol Psychiatry, 2008) [74]

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Herbal formulations such as Ershiwuwei Shanhu Pills (ESP), Lycium barbarum (LB), and Bacopa floribunda (BF) have shown efficacy in mitigating A-induced neurotoxicity, oxidative stress, and mitochondrial dysfunction in BALB/c mice (Hu et al