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The protein was purified through heparin affinity chromatography using 50 mM sodium citrate buffer, pH 6.0, then processed using ion-exchange chromatography (IEC), and finally purified by SEC on a Superdex 75 column (HiLoad 16/600, Cytiva)

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Thus, it has been shown that the inhibition of PARP1 or CD38 increases NAD + levels, leading to an increase in the activity of SIRT1

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