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41 All analyses were performed using GraphPad Prism software (GraphPad Prism Version 10.3, San Diego, CA) and R studio (Version 2024.12.0 + 467)
This metabolic stability contrasts sharply with full-length growth hormone, which can cause glucose intolerance, elevated blood sugar, and insulin resistance (diabetogenic effects)
Measurement of GSSG with or without GSH masking reagent Interference Reducing agents such as ascorbic acid, -mercaptoethanol, dithiothreitol (DTT) and cysteine, or thiol reactive compounds such as maleimide compounds, interfere with the glutathione assay
Examples of pharmaceutical drugs that are considered to have anti-aging properties include Metformin, lithium, and rapamycin (Du et al 2024)
The stress of midlife (and menopause!) is a factor as well