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The washed protein was re-dissolved in 8 M urea (U8020, Solarbio, Beijing, China), and the protein concentration was determined using the BCA assay kit (P0012, Biyun Tian, Shanghai, China) according to the manufacturers instructions 98

Skin Sensitivity: Some individuals may experience mild redness or discomfort at the injection site, which typically subsides shortly after treatment

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Preparing The B12 Injection Site Before administering the injection, it's essential to prepare the injection site properly to minimize the risk of infection and ensure optimal absorption of the B12 solution

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