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As vitamin D is only found in limited natural sources, and its deficiency has become a prevalent health concern worldwide, supplementation has become increasingly crucial

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Los procedimientos de esta investigacin fueron de acuerdo a los lineamientos estipulados por la ley general de la salud en materia de investigacin en seres humanos, donde se aplic el consentimiento informado

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As solvents, aqueous 0.5% formic acid (A), 0.5% formic acid in acetonitrile + 10% water (v/v) (B) and acetonitrile/2-propanol/acetone (1/1/1, v/v/v) (C) were used