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Moreover, a retrospective case-control study revealed that several factors, including advanced age, menopause, weight loss, cyst diameter equal to or exceeding 8.33cm, and the presence of solid areas on ultrasonography, were identified as noteworthy risk factors for EAOC (123)

Cells were seeded into 35 mm dishes (Corning) or 24-well plates (Corning) and allow to grow to confluence in DMEM/F12 medium (Invitrogen 31330038) containing antimycotic/antibiotic (Sigma A5955) and heat-inactivated serum (Gibco 10500064)

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Recent findings indicate that excessive ROS production in HT leads to structural changes in thyroglobulin and thyroid peroxidase, increasing their immunogenicity (Batg et al

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