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Eur J Pharmacol 2000

Patients with epilepsy exhibit increased levels of glycolipid, inflammatory, oxidative, and apoptotic factors, especially patients with generalized seizures (26)

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After cervical dislocation was used to sacrifice the mice, then brain tissues were fixed in 10% formaldehyde solution, embedded in paraffin, and cut into 3 m sections

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The cell suspension was stained using fixable viability violet dye Zombie Red or Fixable Viability Dye eFluor 780 (Invitrogen) for 30 min at 4 C, which was followed by blocking of Fc-receptors with CD16/32 (2.4G2, BD Biosciences) for 15 min at 4 C