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b , For standard skull thinning, a microdrill (not shown) and a microsurgical blade are used to manually thin all three layers of bone until only 30 m of lower cortical bone remains

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Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h

2014;134(2):333e-334e

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Here's what happens in the body after getting an Olympic shot, step by step