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At the same time, a single-center, double-blind, randomized, placebo-controlled trial conducted in Brazil reported negative results

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lactis strains transformed with the different plasmids used in this work were routinely grown at 30 C in M-17 liquid medium (Millipore - Merck KGaA, Darmstadt, Germany) supplemented with 0.5% glucose (Sigma-Aldrich - Merck KGaA, Darmstadt, Germany) (GM-17 medium) without agitation, or in plates containing the same medium solidified with 1.5% agar (Carlo Erba, Cornaredo (MI), Italy)