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The supernatant was discarded, and the cell pellet was resuspended in 300 L of PBS

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Twenty-four hours later, the transfected cells were harvested for unrelated experiments

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Ehrmann DA, Barnes RB, Rosenfield RL, Cavaghan MK, Imperial J

During fasting, methylcitrate levels decreased by 51%, and the ratio of methylcitrate to citrate also exhibited a significant reduction (27%) in the plasma of fasting Pcca -/- (A138T) mice (Fig