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The vitamin is the largest and most structurally complicated vitamin
Samples were separated on SDSPAGE gels, transferred to PVDF membranes and blocked in 2% fatty acid-free BSA and 5% skim milk powder in PBS containing 0.2% Tween-20 (PBST) for lipoate detection, or 5% skim milk powder in PBST for glutaryl-lysine detection
In most cells the GSH concentration is about 12 mM, while in hepatocytes, which export GSH, the concentration can reach about 10 mM
Discontinuation rates due to adverse events were higher in the active treatment groups compared with placebo, particularly at higher doses, though the overall discontinuation rate remained relatively modest
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