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Secondary antibody, 1 :3000 Alexafluor 488 goat-anti-mouse (Invitrogen: Carlsbad, CA) was applied for two hours at room temperature

But depending on your symptoms and what type of anemia your healthcare provider thinks you may have, you may need other tests

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Vitamin B12 deficiency can result in malformed and large red blood cells, known as megaloblastic anemia