The two independent pathways via glutathione reductases in cytosol and class III peroxidases in apoplast could maintain the balance of the GSH/GSSG cycle
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Reverse Transcription-Quantitative Polymerase Chain Reaction The total RNA content was isolated from cells or the tumor using TRIzol reagent (Invitrogen), treated with DNase I (Thermo Fisher Scientific, Cat# EN0521), and then reverse transcribed into cDNA using a Maximal First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat# K1622)
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About this article Cite this article Kim, J., Kim, MJ