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Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously
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At first, injections are given daily or every other day to quickly increase vitamin B12 levels
Setting standards In setting standards we decided that our criteria should be explicit that is, identified before collecting evidence of performance and that our criteria should be appropriate for British general practice, safe, realistic, and achievable
IV bolus without dilution causing hemolysis)
At 10mg/mL, a 5mg dose requires 50 units
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