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To reduce multiplet contamination, we removed barcodes of excessive depth and complexity using upper limits determined by median + 5 median absolute deviation for nCount_RNA and nFeature_RNA

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Conversely, the 3.0 g/d group displayed 4.3% and 6.0% lower values of PP with the 4 th and 5 th sprint and the PP was up to 4.7% lower with the 4.5 g/d dosage

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