For the determination of the enzyme activity in a pH range of 7.09.0, 50 mM Tris-HCl buffer at pH values from 7.0 to 9.0, instead of 50 mM Tris-HCl (pH 8.0), was used
Carnosine acts as a buffer during that decline
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Example: Add 2 mL of bac water to a 5 mg vial to yield 250 mcg per 0.1 mL (or 10 units on an insulin syringe)
Ziegler D., Ametov A., Barinov A., Dyck P
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