After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol
Treating age-related changes in somatotrophic hormones, sleep, and cognition
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Dark chocolate is truly a superfood containing many nutrients including fiber, magnesium, iron, copper, calcium, protein, and manganese
Chemistry Central Journal, 7(1): 15 Li D, Feng Z, Zhou B, Chen H, Yuan R (2022)
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