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In brief, after removal of the perichondrium, dissected growth plates were digested using 2 mg ml 1 collagenase type II in minimum essential medium alpha (MEM) GLUTAMAX supplemented with 100 units per ml penicillin, 50 g ml 1 streptomycin and 10% FBS (all from Gibco, ThermoFisher Scientific) for 3 h at 37 C

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Individual results with any supplement vary based on baseline health status, dietary patterns, lifestyle factors, and genetic variables