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Instead of letting a free flow of blood to cells, the blood brain barrier only lets in specific amounts of specific nutrients

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Liver samples were thoroughly homogenized for 30 s in 500 L of 20 mM phosphate buffer (equimolar mix of K 2 HPO 4 and KH 2 PO 4 ) and then 400 L were mixed with 1 mL of a 1:1 v/v chloroform/methanol mixture, vortexed and then samples were rotated in a wheel for 60 min at RT for 30 min

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