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The active metabolite, 1,25-dihydroxyvitamin D3 (calcitriol), is generated via sequential hydroxylations in the liver (by CYP2R1) and kidneys (by CYP27B1) (80)

S4762, Sigma-Aldrich, Germany) for 10 minutes at room temperature, and then incubated 10 minutes in biotin-concanavalin A (0.2 mg/ml in 1 PBS, Sigma-Aldrich) at room temperature 21

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It inhibits tyrosinase (the key enzyme that makes dark melanin) and shifts pigment synthesis toward lighter pheomelanin

AOD 9604 does not cause fluid retention

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