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We used unpaired one-way ANOVA analysis (ANOVA) to assess the significance of differences in mean values between experimental groups, and Tukey's posthoc test was used to adjust multiple comparisons between experimental groups

In the first and second stages, lab testing will show declining levels of vitamin B12 and holotranscobalamin (transporter of B12), while MMA and homocysteine levels will remain within or around the normal range