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This method is based on the color reaction between allyl thiourea and potassium ferricyanide solution in dilute acetic acid which measures the optical density of the color complex at 600 nm and it works in the range of 2.5 to 7.5 picogram of AITC per milliliter of the color solution

While many of the studies used animal models and have not been proven in humans yet, clinical trials are ongoing

For context, the toxic dose is estimated at roughly 22,500 mg, compared to a typical weekly dose of about 6 mg

The mixture was incubated on ice for 30 minutes

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