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This is the conservative recommendation based on preservative effectiveness and peptide stability data

Male, 18-week-old C57BL/6J mice, maintained on a 60% high-fat diet (HFD

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[DOI] [PMC free article] [PubMed] [Google Scholar] 31.Attenuation of synaptic toxicity and MARK4/PAR1-mediated tau phosphorylation by methylene blue for Alzheimer's disease treatment

The depletion of brain glutathione has been linked to various neurological conditions, emphasizing the importance of maintaining adequate levels of this antioxidant for neurological health 2

Protein digestion For cell lysates, 60 g of protein per sample were reduced, alkylated, and digested