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Glutathione, GSH307.325 g/mol189193 C-SH 1 2G-SHG-S-S-G2 NADPH R-SH0.510 mmol/L 4 GSHGSSG100:1GSH371mg L-1HIV 5 7 GSH CAS 70-18-87 PubChem 745-1248867 C10H17N3O6S7 307.325g/mol7 189193 7 7 7 7 1001000 mg/100 g 26-34 mg/100 g58-73 mg/100g10-15 mg/100 g12-33 mg/100 g0.06-0.7 mg/100 g 1 70 mg/100 GPxH2O2H2OGSSGGSHCE 9 -S-GSTGSH 10 NKNF-B-3-GSHGSH 11 GSH-3-SH-GSH-SHGSHIB/NF-BGSH 12 13 20 DTNB HPLCGSH 21 DTNB DTNB5--2-412 nmDTNB400 nmDTNB412 nm 21 1DTNB0.01 mol/LDTNB0.05 mol/LpH7.0DTNB 2DTNBDTNB0.5 mol/LpH8.0Tris-HCl100DTNB 30.13.0 mol/L0.5 mL1.5 mL0.15 mol/LNaOH3%0.5 mLpH8.02520.5 mLDTNB255412 nm 21 0.05 mg10 mL2% HPO3100 mL100 mL5 mL100 mL2% HPO35 mL10 mL5% 1 mL20.001 mol/L0.001 mol/L2.445 mL100% V-0.001 mol/LmL2.445 -100%mL HPLC 21 Kromasil C184.6 mm 250 mm5 m 3.0 g1.0 g500 mLpH3 = 964 210 nm0.8 mL/min3010 L 25 g/mL50 g/mL100 g/mL200 g/mL400 g/mL800 g/mL 25800 g/mL 10 L0.9995 10 L HPLC 0.1 g0.3 g0.6 g 2-3-SH 22 22 1310.4 g4121 1 22 1500 mg/m2100 ml5%15251600 mg151 mg35 mg 22 2 1200 mg1130 12002400 mg1130 1200 mg1130 1800 mg1130 1800 mg111430 12001800 mg111430 22 31500 mg/m2 4500 mg/m2100 ml1300600 mg 5300600 mg20 ml 1 22 2 3 1 22 2 3 4 5 1

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1.2 Pathogenesis of hashimotos thyroiditis Hashimotos thyroiditis (HT) is characterized by dense lymphocytic infiltration of the thyroid gland, leading to progressive destruction of follicular cells