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Glucose level determines excitatory or inhibitory effects of adiponectin on arcuate POMC neuron activity and feeding

In instances when deficiencies exist and it is difficult to replace such deficiencies with supplements alone, one can receive injections and intravenous replacement

cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

Group 2 was diabetic untreated (DU)

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(May 2008)