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Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate

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Furthermore, our Family Health Care team provides personalized nutritional counseling and vitamin B12 injections for patients who follow strict vegetarian or vegan diets, as these dietary patterns often lack sufficient vitamin B12 naturally present in animal-based products

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This effect was confirmed in vivo as seen by reduced tumor burden in mouse xenografts