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Name: positive side effects of tirzepatide The promise tirzepatide: A narrative review of metabolic benefits Tirzepatide Side Effects: Common &
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150 mL of 2.0 mol / L hydrogen chloride ethyl acetate solution was added to react for 2 h

For purification, cell pellets were resuspended in 35 ml of lysis buffer (50 mM Tris, pH 7.5, 300 mM NaCl, 10 mM imidazole, 1 mM dithiothreitol (DTT), 0.5% Triton X-100, and one tablet of protease inhibitor cocktail per 50 ml), sonicated for 1 min (1 s on, 5 s off), and centrifuged at 87,000 g for 20 min