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Analysis of oxidative stress-induced cellular senescence Cell senescence assay was conducted with Senescence -Galactosidase Staining Kit (Cell Signaling Technology) according to the manufacturers instruction

Plates were sealed and placed in a vertical position into a controlled growth chamber (VB1514 Vtsch Industrietechnik) under a 16 h photoperiod (75 mol m 2 s 1 ) at 22 C/18 C for 10 days

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The plates were then incubated at 37 C and 5% CO2 for 14 days

These experiments demonstrate significant protein association with gel homogenates harboring GSH compared to controls