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Nature 485 , 242245 (2012)

PMID: 71 HowardEHurrellBPHelouDGShafiei-JahaniPHasiakosSPainterJet al

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Too little signal and cells barely respond

the solution is cooled to about 1C (-2 to 4C) and p -toluenesulfonyl chloride (1.0 kg) is added and then triethylamine (0.82 kg) at no more than 10C is slowly added (over about 3-6 hours in an exothermic reaction)

Compared to earlier analogs, GHRP-2 provides intense somatotropic stimulation with more moderate activation of central orexigenic (appetite-stimulating) pathways, allowing researchers to study growth hormone surges in isolation from extreme systemic metabolic flux