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Bilirubin ameliorates osteoarthritis via activating Nrf2/HO-1 pathway and suppressing NF-B signalling

Ideally, the injection is administered into a fatty layer of skin, such as: back of arm, abdomen, or flank/lumbar region

2.9 Flow cytometric cell sorting analysis Following the procedure described in Section 2.2, HUVEC cells were digested and collected using trypsin without EDTA