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Alternatively, if there is still quite a bit of your protein in the flow-through then you could use even more resin next time to capture more of your target protein (Figure 5, right)

Temperature cycling, repeatedly moving between refrigerator and room temperature, accelerates degradation beyond what either constant temperature would cause

Rahban, R

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Organelle clearance, primarily the expulsion of mitochondria in erythroblasts or reticulocytes, is accompanied by the removal of the mitochondrial apoptosis-associated machinery

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