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Cytochrome c release assay Cells were fractionated to obtain cytoplasmic and mitochondria-enriched fractions using Dounce homogenization and differential centrifugation as per the Abcam cytochrome c release kit (ab65311)

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27 Iron overload appears to be the main driver of ferroptosis and has been shown to make many cell types more susceptible to this form of cell death

While B12 injections can work wonders, theyre not a standalone solution