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One such intermediate byproduct is a potent toxin known as acetaldehyde. While acetaldehyde is short-lived, usually existing in the body only for a brief time before it is further broken down into acetate, according to a NIAAA fact sheet, it has the potential to cause significant damage to your cells and tissues in the liver, pancreas, brain and elsewhere

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After the incubation was completed, the cells were washed three times with PBS solution, and the ROS levels were detected and analyzed by flow cytometry

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This is the same standard used by hospitals and private practices