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9 Deletion of Bmal1 leads to impaired glucose tolerance on a chow diet, which is prevented by NDI1, related to Fig

Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

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Oxidative damage and protein nitration are involved in the pathogenesis of MC Research revealed that the positive immune markers of 3-nitrotyrosine (3-NT), 4-hydroxynonenol (4-HNE), and malondialdehyde (MDA) are significantly increased in a pig model of MC, among which 4-HNE and MDA have been identified as robust markers of oxidative stress, while 3-NT is a biomarker of endogenous peroxynitrite activity