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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]

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These pathways are established drivers of HCC, linking growth signals to cell proliferation, survival, and metabolism [46, 47]

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All of these factors contributed to higher levels of serum UA and indicated that elevated UA levels can be viewed as an evolutionary advantage rather than as a waste product