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Measurement of Serum Testosterone Levels Serum testosterone concentrations were measured using a competitive enzyme-linked immunosorbent assay (ELISA) kit (Elabsciences, Cat N: E-EL-0155, USA) according to the manufacturers instructions

In addition, the dual InsR/IGF-IR inhibitor linsitinib can prevent the emergence of breast cancer cells that are resistant to long-term estrogen deprivation or antiestrogen treatment and inhibit growth of established ER+ breast cancer xenografts in ovariectomized mice [107]

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Calculation of EC 50 Biological responses to ligand concentrations typically follow a sigmoidal function

Possible Health Benefits: It strengthens the immune system

Some patients in these categories may still be candidates with close monitoring