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Other antioxidants such as vitamin C that appear to work work synergistically with glutathione are often included

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Acetamide (A108460-25kg, Aladdin, Shanghai, China) was then added to achieve a concentration of 11 mM and incubated at room temperature for 15 min

Nat Commun 12:16 Karunatilleke NC, Fast CS, Ngo V, Brickenden A., Duennwald ML, Konermann L, Choy WY (2021) Nrf2, the major regulator of the cellular oxidative stress response, is partially disordered

Do not vortex or shake vigorously, as this can disrupt the copper-peptide coordination complex