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The following mutants were generated: for p97-ND1LP246T, P247A, P247K, K251A, D304N, E305Q, K312A, K312E, K312R, K312R-R313A, R313A, R313A-E314R, K315A, N348Q, R349A, R359A, R359A-R362A, R359K, F360A, Cys-F360C-A413C (Cys: C69V-C77V-C105A-C174A-C184W-C209V-C415A), F360P and R362A

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After an additional twenty-four hours, cells were detached by trypsinization, washed in PBS and frozen until lipids were extracted