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For adults, this is a BMI of 30 or more or of 27 or more with at least one weight-related medical condition, such as Type 2 diabetes, sleep apnea, high cholesterol, or high blood pressure
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For hepatocyte-specific Gdh1 deletion, Glud1 fl/fl mice received a tail-vein injection of AAV8-TBG-GFP (Addgene, 105535) or AAV8-TBG-Cre (1.5 10 11 genome copies per mouse
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