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Preparing reagents for calcium imaging For calcium imaging of the AWA neurons in response to diacetyl, we used two types of solutions: Stimulus and Buffer. Both solutions were composed of a CTM buffer supplemented with 10 mM Levamisole (Sigma, CAS Number: 16595805)

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GLT1 then transfers the amide group from glutamine to -ketoglutarate, synthesising two molecules of glutamate 18