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For people over 60, the need for glutathione increases significantly

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Though the mechanism of action of antidepressants amongst patients with fibromyalgia has not been systematically studied, there has been evidence for both serotonergic and noradrenergic transmission in fibromyalgia [38]

Standard or sample was transferred in triplicate to a 96-well plate and GSH was then derivitized by the addition of 10 mM 2,3-naphthalenedicarboxyaldehyde and the fluorescence at Ex/Em wavelength of 472/528 nm was quantified using a fluorescent plate reader (Synergy 2, BioTek)

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