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Preparative cultures (1 l) for each transformed strain were incubated with shaking at 37C in lysogeny broth (LB) supplemented with 5 mM AcK (A4021-5G Sigma), 10 M sodium selenite (10102-18-8, AlfaAesar), and appropriate antibiotics (100 g/ ml ampicillin (BP1760-25, Fisher) for pET and 34 g/ ml chloramphenicol (02930-100G, Ampresco) for pTech)

We can't stress this enough: you're no longer working with the compound you intended to study

Average recovery: 2.3 months of impaired sight

The combined preparation is sold strictly for laboratory research use only and is not represented as the FDA-approved finished drug product or as any approved therapeutic

Codazzi, F

Zhai, H., & Maibach, H