As an added precaution for patients with a reaction history within the previous five years, which makes them higher risk, a two-step DOC, starting with 10% of the target dose, is recommended
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We recommend starting with a subcutaneous protocol
Then the cells were centrifuged and washed once with 1 ml of 0.1 M borax (Sigma-Aldrich, 71997), followed by another wash with 1% BSA in PBS
