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Sample size Previously reported results [12] were adopted in two-way ANOVA sample size calculation using Statistica 13.1 software (Dell Inc., Tulsa, OK, USA)

Genetic screening of mtDNA mutations follows the criteria defined by DiMauro and Schon, 71 considering the presence of neutral polymorphisms, evolutionarily conserved and phenotypically distinct mutations, deletion mutations exhibiting heteroplasmy, and how the degree of heteroplasmy aligns with the severity of symptoms

Diabetes 58(4):975983 Nystrm T, Gutniak MK, Zhang Q, Zhang F, Holst JJ, Ahrn B, Sjholm A (2004) Effects of glucagon-like peptide-1 on endothelial function in type 2 diabetes patients with stable coronary artery disease