We thank Dirk Schnappinger and Sabine Ehrt (Weill Cornell Medical College, New York) for kindly providing the cloning vectors used in the Gateway assembly of the Rv1127c expression plasmid
HUBERMAN: In other words, you want cortisol levels pretty high, in the early part of the day, but you want them very, very low in the later part of the day and at night
For immunohistochemical staining, we incubated the sections with primary antibodies overnight at 4 C and used a horseradish peroxidase-streptavidin detection system (Dako) to detect immunoactivity followed by counter-staining with hematoxylin (Sigma-Aldrich)
This variability suggests that the presence of a mutant RET tyrosine kinase receptor is necessary but not sufficient for transformation
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